Agaricus bisporus Lectin (ABA/ABL) - Pure
Regular price
$179.50
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SKU# 21510154-1
Product Description
Agaricus bisporus agglutinin/lectin (ABA/ABL) is isolated from white button mushrooms. It has a molecular weight of 58,500 as determined by gel filtration that corresponds to a tetramer, and has an isoelectric point that ranges between pH 5.0 and pH 6.0. ABA is a mixture of two phytohemagglutinins (PHA) with similar specificities for carbohydrate (PHA-A and PHA-B). Each monomer has 2 distinct carbohydrate binding sites, one for galactose-ß≤-1,3-N-acetylgalactosamine and another for galactose-ß≤-1,3-N-acetylglucosamine, but does not bind monosaccharides.
ABA has a non-specific blood group recognition since PHA-A and PHA-B agglutinate erythrocytes independent of their blood group type. The O-linked glycopeptide released by trypsin is a potent inhibitor of both the isolectins. Removal of the terminal sialic acid residue from the glycopeptide increases its inhibitory potency by 8-fold. Periodate or ß≤-galactosidase treatment of the trypsin released glycopeptide destroys all inhibitory activity. Simple sugars are very poor inhibitors of these isolectins. However, a galactose residue appears to play a major role in these lectin binding mechanisms. The sugar linkage is also important since a synthetic N-linked glycopeptide is not inhibitory.
ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins. After internalization, it inhibits nuclear import of nuclear localization signal-dependent proteins. See other ABA conjugates.
This product comes in lyophilized form and is stored below -20ºC.
Technical Specifications
| Molecular Weight | : | 58.5 kDa |
| Synonyms | : | ABA/ABL |
| Appearance Form | : | White to off-white |
| Shelf Life | : | 2 years |
| Storage Temperature | : | -20°C |
| Hazardous Shipping | : | Non-hazardous |