Sambucus nigra Lectin (SNA/EBL I+II) - Pure
Regular price
$140.00
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SKU# 21511172-1
Product Description
Affinity purified Sambucus nigra lectin (EBL I+II, also known as SNA) is is composed of two ricin-related lectins. EBL I+II is a tetrameric glycoprotein consisting of four disulfide-linked chains, two of about 36,000D and two of about 38,000D with a recognition of the Neu5Ac(a2-6)Gal or GalNAc linkage in glycoconjugates.
EBL I+II is not blood group specific but it does agglutinate type A erythrocytes more strongly than either B or O cells. Trypsin treated erythrocytes react better than untreated cells. Neuraminidase treated erythrocytes react with this lectin due to the presence of terminal galactose resides. SNA has a broad specificity for both ?- and ?-linked galactose, as well as unrelated sugars such as fucose. It has been determined that the lectin does not bind to glycoproteins or glycolipids containing only terminal ?(2,3)-linked sialic acid residues.
EBL I is composed of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. EBL II consists only of carbohydrate-binding B-chains. EBL I+II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems independent from the catalytic activity of the A-chain, but depends on the carbohydrate binding B-chain.
Technical Specifications
| Molecular Weight | : | 140 kDa |
| Synonyms | : | SNA/EBL I+II |
| Shelf Life | : | 2 years |
| Storage Temperature | : | -20°C |
| Hazardous Shipping | : | Non-hazardous |