Succinylated Triticum vulgaris Lectin (Succ WGA) - Pure
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$154.05
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SKU# 30330042-1
Product Description
Succinylated wheat germ agglutinin (Succ-WGA) is isolated from wheat germ (Triticum vulgaris) and is a derivative from the native wheat germ agglutinin (WGA). It has a molecular weight of 36,000 and is an acidic protein with an isoelectric point of pH 4.0 +/- 0.2. It is dimeric at pH down to 5 with a dissociation that occurs at pH lower than 4.5. Succ-WGA agglutinates rabbit erythrocytes at a minimal concentration of about 2 µg/ml, and also agglutinates human erythrocytes of blood groups A, B, and O types. It has a carbohydrate specificity towards GlcNAc and elutes a concentration of chitin hydrolysate or GlcNAc with acid or salt.
The solubility of Succ-WGA is around 100 times higher than the unmodified lectin at neutral pH. Studies suggests that Succ-WGA does not bind to sialic acid residues, unlike WGA, but retains its specificity towards N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing N-acetylglucosamine structures.
This product comes in a lyophilized form and is stored at -20ºC. Calcium is required for binding.
Technical Specifications
| Molecular Weight | : | 36 kDa |
| Synonyms | : | WGA |
| Shelf Life | : | 2 years |
| Storage Temperature | : | -20°C |
| Hazardous Shipping | : | Non-hazardous |