Glutathione Separopore® 4B-CL

Product Description

Applications:

Reduced glutathione is covalently linked Separopore^® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Glutathione Separopore^® Super Flow (SF) is used for protein purification by batch/column and scale-up procedures. Glutathione Separopore^® Super Flow (SF) has an exellent protein binding capacity. The good flow properties of the 92 ‚Äö√¢√†‚àö‚↮‚Äö√†¬¥m (average size) highly crosslinked Separopore^® matrix makes an excellent choice for protein purification by column purification or batch process for scale-up.

This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated 4% crosslinked agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity of 5 mg or more of Glutathione-S-transferase (GST) per ml of wet gel. Bound GST-fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Storage: 2 ¬∫C - 8 ¬∫C